Using VectorNTI

VectorNTI is a commercial suite for analyzing DNA and protein sequences, retrieving information from GenBank, designing Oligos and so forth.

In this tutorial we will be using VectorNTI version 7 for Windows.. Download for PC or Mac from the Informax, Inc site. Wherever possible I will try and include information for Mac users where it is different from PC users.

In this module we will go through registering your copy of VectorNTI using the Dynamic License Sever, opening files, editing DNA molecules, generating restriction maps, plasmid maps, in silico cloning, PCR primer design. Annotating sequences and saving in different formats.

Registering VectorNTI

If you have just installed a copy of VectorNTI either by downlaoding it or copying from a CD, you will be asked to register your software. UT provides a dynamic license server for your use. Currently the server is limited to 5 simultaneous connections. If there are 5 open connections you will not be allowed to use the software, and will have to wait for a connection to become available.

There are steps to licensing the software (but you don't have to do these on a Mac, see below):

  1. Open Vector NTI suite license manager.
  2. For all applications select "Dynamic License" from the pull down menu
  3. Under Dynamic License settings enter a name for the computer. This will be used so we know who is accessing the machine.
  4. In URL of DLS enter the following information http://cgb.utmem.edu/cgi-bin/vntidls.cgi
  5. Click Close
  6. You may now use vectorNTI, start it from the START menu in Windows
For Mac users, just start VectorNTI and when the License Server Connection window opens enter the above URL and press the Connect button. You may now use vectorNTI.


In the following modules, we will figure out how to use vectorNTI. These are broken down as follows:

  1. Getting started. Using databases and subbases.
  2. Using the vectorNTI DNA display.
  3. Designing primers for PCR in vectorNTI.
The assignment for Module 4 is now online.