In this class we will talk about genomic sequencing and sequencing in general.Many of the same techniques apply whether you are sequencing a single clone or an entire genome.
The links for this class are:
Download these sequences (Note this is the PC version -- the mac version will be available shortly).DONOT TRIM ANY SEQUENCES OR VECTOR. JUST ASSEMBLE THE SEQUENCES.
Assemble these sequences using your favorite assembler, phred/phrap, sequencher, or contig express.
NOW DISSOLVE ALL THE CONTIGS. TRIM THE ENDS AND THE VECTOR You will need pZero2, which is also in this Sequencher VecBase file.
Reassemble the sequences