In this class we will talk about genomic sequencing and sequencing in general.Many of the same techniques apply whether you are sequencing a single clone or an entire genome.

The links for this class are:

- Overview
- Beginning Sequencher
- Sequencher - chromatograms
- Sequencher - trimming sequence
- Sequencher - assembly
- Artemis

**Also:**

- Download the Sequencher demo (utmem.edu and windows only)
- Download some sequences to import
- Download the Java Runtime environment (utmem.edu and windows only)
- Download Artemis (utmem.edu and windows only)
- Download the consensus sequence

Download these sequences (Note this is the PC version -- the mac version will be available shortly).

Assemble these sequences using your favorite assembler, phred/phrap, sequencher, or contig express.

- How many contigs did you get?
- How many sequences are not in contigs?
- What is the longest contig?
- What is the shortest contig?
**NOW DISSOLVE ALL THE CONTIGS. TRIM THE ENDS AND THE VECTOR**You will need pZero2, which is also in this Sequencher VecBase file.Reassemble the sequences

- How many contigs did you get?
- How many sequences are not in contigs?
- What is the longest contig?
- What is the shortest contig?
- Which assembly program did you use?